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pcDNA5FRT/TO-UBE2QL1-CS-Strep/HA
(Plasmid #129513)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129513 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5-FRT-TO-GW-cSH
  • Backbone manufacturer
    Ruedi Aebersold
  • Backbone size w/o insert (bp) 7017
  • Total vector size (bp) 5827
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    UBE2QL1 C88S
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    485
  • GenBank ID
    NM_001145161.3
  • Entrez Gene
    UBE2QL1
  • Promoter CMV
  • Tag / Fusion Protein
    • 2xStrep/HA (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5FRT/TO-UBE2QL1-CS-Strep/HA was a gift from Hemmo Meyer (Addgene plasmid # 129513 ; http://n2t.net/addgene:129513 ; RRID:Addgene_129513)

  • For your References section:

    The ubiquitin-conjugating enzyme UBE2QL1 coordinates lysophagy in response to endolysosomal damage. Koerver L, Papadopoulos C, Liu B, Kravic B, Rota G, Brecht L, Veenendaal T, Polajnar M, Bluemke A, Ehrmann M, Klumperman J, Jaattela M, Behrends C, Meyer H. EMBO Rep. 2019 Aug 21:e48014. doi: 10.15252/embr.201948014. 10.15252/embr.201948014 PubMed 31432621
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