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Addgene

LifeAct-mAvicFP1
(Plasmid #129512)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129512 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pC1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LifeAct-mAvicFP1
  • Mutation
    mAvicFP1 is AvicFP1 F64L + A206K
  • Promoter CMV

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LifeAct-mAvicFP1 was a gift from Nathan Shaner (Addgene plasmid # 129512 ; http://n2t.net/addgene:129512 ; RRID:Addgene_129512)

  • For your References section:

    Aequorea's secrets revealed: New fluorescent proteins with unique properties for bioimaging and biosensing. Lambert GG, Depernet H, Gotthard G, Schultz DT, Navizet I, Lambert T, Adams SR, Torreblanca-Zanca A, Chu M, Bindels DS, Levesque V, Nero Moffatt J, Salih A, Royant A, Shaner NC. PLoS Biol. 2020 Nov 2;18(11):e3000936. doi: 10.1371/journal.pbio.3000936. eCollection 2020 Nov. 10.1371/journal.pbio.3000936 PubMed 33137097
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