H2B-mAvicFP1
(Plasmid
#129511)
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PurposeExpresses H2B-mAvicFP1 in mammalian cells (human histone 2B)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 129511 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepC1
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Backbone manufacturerClontech
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameH2B-mAvicFP1
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Alt nameHistone 2B
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SpeciesH. sapiens (human)
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MutationmAvicFP1 fused to the C terminus of human histone 2B
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
H2B-mAvicFP1 was a gift from Nathan Shaner (Addgene plasmid # 129511 ; http://n2t.net/addgene:129511 ; RRID:Addgene_129511) -
For your References section:
Aequorea's secrets revealed: New fluorescent proteins with unique properties for bioimaging and biosensing. Lambert GG, Depernet H, Gotthard G, Schultz DT, Navizet I, Lambert T, Adams SR, Torreblanca-Zanca A, Chu M, Bindels DS, Levesque V, Nero Moffatt J, Salih A, Royant A, Shaner NC. PLoS Biol. 2020 Nov 2;18(11):e3000936. doi: 10.1371/journal.pbio.3000936. eCollection 2020 Nov. 10.1371/journal.pbio.3000936 PubMed 33137097