pNCST-AvicFP4
(Plasmid #129507)

• Purpose: Expresses AvicFP4 constitutively in E. coli (most strains)
• Depositing Lab: Nathan Shaner
• Publication: Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. doi: 10.1371/journal.pbio.3000936. eCollection 2020 Nov.

Backbone

• Vector backbone: pNCST
• Backbone size w/o insert (bp): 2900
• Total vector size (bp): 3600
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AvicFP4
• Alt name: Aequorea victoria FP4
• Species: Aequorea victoria
• Mutation: One of two variants of AvicFP4 tested with essentially identical optical properties
• Promoter: synthetic constitutive (stationary phase) promoter + T7 promoter upstream

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• Supplemental Documents:
  • pNCST-AvicFP4.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Expression from this vector is best in fast-growing E. coli strains.