pNCST-AvicFP2
(Plasmid
#129505)
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PurposeExpresses AvicFP2 constitutively in E. coli (most strains)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 129505 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepNCST
- Backbone size w/o insert (bp) 2900
- Total vector size (bp) 3600
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAvicFP2
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Alt nameAequorea victoria FP2
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SpeciesAequorea victoria
- Promoter synthetic constitutive (stationary phase) promoter + T7 promoter upstream
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Expression from this vector is best in fast-growing E. coli strains.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNCST-AvicFP2 was a gift from Nathan Shaner (Addgene plasmid # 129505 ; http://n2t.net/addgene:129505 ; RRID:Addgene_129505) -
For your References section:
Aequorea's secrets revealed: New fluorescent proteins with unique properties for bioimaging and biosensing. Lambert GG, Depernet H, Gotthard G, Schultz DT, Navizet I, Lambert T, Adams SR, Torreblanca-Zanca A, Chu M, Bindels DS, Levesque V, Nero Moffatt J, Salih A, Royant A, Shaner NC. PLoS Biol. 2020 Nov 2;18(11):e3000936. doi: 10.1371/journal.pbio.3000936. eCollection 2020 Nov. 10.1371/journal.pbio.3000936 PubMed 33137097