Zebrafish PAC (fPAC)
(Plasmid
#129498)
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PurposeExpresses zebrafish TMEM206. In pcDNA3.1(+) backbone
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 129498 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1(+)
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Backbone manufacturerGenScript
- Backbone size w/o insert (bp) 5400
- Total vector size (bp) 6477
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Modifications to backboneInsert zebrafish PAC
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namezebrafish PAC
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Alt namefPAC
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Alt nameTMEM206
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SpeciesD. rerio (zebrafish)
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GenBank IDNP_001278691
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Entrez Genepacc1 (a.k.a. PAC, fPAC, tmem206, zgc:63579)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer CMV - F
- 3′ sequencing primer BGH - Rev (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byGene was cloned and received from GenScript
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Zebrafish PAC (fPAC) was a gift from Zhaozhu Qiu (Addgene plasmid # 129498 ; http://n2t.net/addgene:129498 ; RRID:Addgene_129498) -
For your References section:
PAC, an evolutionarily conserved membrane protein, is a proton-activated chloride channel. Yang J, Chen J, Del Carmen Vitery M, Osei-Owusu J, Chu J, Yu H, Sun S, Qiu Z. Science. 2019 Apr 26;364(6438):395-399. doi: 10.1126/science.aav9739. Epub 2019 Apr 25. 10.1126/science.aav9739 PubMed 31023925