pcDNA3.1+/-FLI1-C‐(K)‐DYK
(Plasmid
#129497)
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PurposeMammalian expression of human FLI1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 129497 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1(+)
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFLI1
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SpeciesH. sapiens (human)
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Entrez GeneFLI1 (a.k.a. BDPLT21, EWSR2, FLI-1, SIC-1)
- Promoter CMV
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Tag
/ Fusion Protein
- Flag (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byCloned by GenScript
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1+/-FLI1-C‐(K)‐DYK was a gift from Marjorie Brand (Addgene plasmid # 129497 ; http://n2t.net/addgene:129497 ; RRID:Addgene_129497) -
For your References section:
Single-Cell Proteomics Reveal that Quantitative Changes in Co-expressed Lineage-Specific Transcription Factors Determine Cell Fate. Palii CG, Cheng Q, Gillespie MA, Shannon P, Mazurczyk M, Napolitani G, Price ND, Ranish JA, Morrissey E, Higgs DR, Brand M. Cell Stem Cell. 2019 May 2;24(5):812-820.e5. doi: 10.1016/j.stem.2019.02.006. Epub 2019 Mar 14. 10.1016/j.stem.2019.02.006 PubMed 30880026