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pIRES2-hPAC
(Plasmid #129488)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129488 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRES2-EGFP
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5300
  • Total vector size (bp) 6353
  • Modifications to backbone
    Inserted human PAC using XhoI and EcoRI restriction enzyme sites.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Transmembrane protein 206
  • Alt name
    TMEM206
  • Alt name
    C1orf75
  • Alt name
    hPAC
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1053
  • GenBank ID
    55248 NP_060722
  • Entrez Gene
    PACC1 (a.k.a. C1orf75, PAC, TMEM206)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer CMV - F
  • 3′ sequencing primer IRES Rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIRES2-hPAC was a gift from Zhaozhu Qiu (Addgene plasmid # 129488 ; http://n2t.net/addgene:129488 ; RRID:Addgene_129488)

  • For your References section:

    PAC, an evolutionarily conserved membrane protein, is a proton-activated chloride channel. Yang J, Chen J, Del Carmen Vitery M, Osei-Owusu J, Chu J, Yu H, Sun S, Qiu Z. Science. 2019 Apr 26;364(6438):395-399. doi: 10.1126/science.aav9739. Epub 2019 Apr 25. 10.1126/science.aav9739 PubMed 31023925
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