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Addgene

EGFP-hSV1_WT
(Plasmid #129483)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129483 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4731
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Supervillin
  • Alt name
    Supervilin, isoform 1
  • Alt name
    SVIL
  • Alt name
    hSV1
  • Species
    H. sapiens (human)
  • Entrez Gene
    SVIL (a.k.a. MFM10)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer EGFP-C
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EGFP-hSV1_WT was a gift from Elizabeth Luna (Addgene plasmid # 129483 ; http://n2t.net/addgene:129483 ; RRID:Addgene_129483)

  • For your References section:

    Novel interactors and a role for supervillin in early cytokinesis. Smith TC, Fang Z, Luna EJ. Cytoskeleton (Hoboken). 2010 Jun;67(6):346-64. 10.1002/cm.20449 PubMed 20309963
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