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pAAV/EF1a::DIO-iLMO2
(Plasmid #129482)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129482 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-Ef1a-DIO eNpHR 3.0-EYFP
  • Backbone manufacturer
    Karl Deisseroth (Addgene plasmid # 26966)
  • Backbone size w/o insert (bp) 5590
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    iLMO2
  • Alt name
    inhibitory luminopsin 2
  • Alt name
    eNpHR2.0-Nanolantern
  • Species
    N. pharaonis, A. victoria, R. reniformis
  • Insert Size (bp)
    2527
  • Promoter EF1a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site NheI (not destroyed)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pAAV-CaMKIIa-eNpHR3.0-EYFP from Karl Deisseroth, Stanford U (Addgene #26971) and Nano-lantern/pcDNA3 from Takeharu Nagai, Osaka U (Addgene #51970)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

iLMO2 (NpHR-Nanolantern-ER export motif) from pcDNA3 vector was inserted in the inverse orientation using AscI/NheI

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV/EF1a::DIO-iLMO2 was a gift from Robert Gross (Addgene plasmid # 129482 ; http://n2t.net/addgene:129482 ; RRID:Addgene_129482)

  • For your References section:

    Motoneuron activity is required for enhancements in functional recovery after peripheral nerve injury in exercised female mice. Jaiswal PB, Tung JK, Gross RE, English AW. J Neurosci Res. 2017 Aug 3. doi: 10.1002/jnr.24109. 10.1002/jnr.24109 PubMed 28771790
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