FUiLMO2W
(Plasmid #129480)

• Purpose: 3rd-generation lentiviral vector for iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemical
• Depositing Lab: Robert Gross
• Publication: Tung et al Sci Rep. 2015 Sep 24;5:14366. doi: 10.1038/srep14366.

Backbone

• Vector backbone: FUGW
• Backbone manufacturer: David Baltimore (Addgene plasmid # 14883)
• Backbone size w/o insert (bp): 9180
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Bleomycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: iLMO2
• Alt name: inhibitory luminopsin 2
• Alt name: eNpHR2.0-Nanolantern
• Species: N. pharaonis, A. victoria, R. reniformis
• Insert Size (bp): 2526
• Promoter: human ubiquitin C

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pAAV-CaMKIIa-eNpHR3.0-EYFP from Karl Deisseroth, Stanford U (Addgene #26971) and Nano-lantern/pcDNA3 from Takeharu Nagai, Osaka U (Addgene #51970)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

iLMO2 (NpHR-Nanolantern-ER export motif) from pcDNA3 vector was inserted using BamHI/EcoRI