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pAAV/CBA::EGFP-P2A-tC3-FLAG
(Plasmid #129477)

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Item Catalog # Description Quantity Price (USD)
Plasmid 129477 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV/CBA::EGFP
  • Backbone size w/o insert (bp) 5360
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLAG-tagged (C-terminus) truncated (AA173-201) exoenzyme C3 from C. botulinum, P2A, EGFP
  • Species
    C. botulinum, A. victoria, porcine teschovirus-1
  • Insert Size (bp)
    179
  • Promoter CBA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsrGI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer GFP-fwd
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

29 aa of C3 transferase tagged with FLAG at the C-terminus was inserted downstream of EGFP following self-cleaving P2A in-frame using BsrGI/HindIII

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV/CBA::EGFP-P2A-tC3-FLAG was a gift from Robert Gross (Addgene plasmid # 129477 ; http://n2t.net/addgene:129477 ; RRID:Addgene_129477)
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