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Addgene

FUG-P2A-tC3-W
(Plasmid #129475)

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Item Catalog # Description Quantity Price (USD)
Plasmid 129475 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FUGW
  • Backbone manufacturer
    David Baltimore (Addgene plasmid # 14883)
  • Backbone size w/o insert (bp) 9916
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Bleomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    truncated (AA173-201) exoenzyme C3 from C. botulinum, P2A, EGFP
  • Species
    C. botulinum, A. victoria, porcine teschovirus-1
  • Insert Size (bp)
    154
  • Promoter human ubiquitin C

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsrGI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

29 aa of C3 transferase was inserted downstream of EGFP following self-cleaving P2A in-frame using BsrGI/EcoRI

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FUG-P2A-tC3-W was a gift from Robert Gross (Addgene plasmid # 129475 ; http://n2t.net/addgene:129475 ; RRID:Addgene_129475)
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