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pcDNA3-iLMO2
(Plasmid #129474)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129474 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5382
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    iLMO2
  • Alt name
    inhibitory luminopsin 2
  • Alt name
    eNpHR2.0-Nanolantern
  • Species
    N. pharaonis, A. victoria, R. reniformis
  • Insert Size (bp)
    2546
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-fwd
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pAAV-CaMKIIa-eNpHR3.0-EYFP from Karl Deisseroth, Stanford U (Addgene #26971) and Nano-lantern/pcDNA3 from Takeharu Nagai, Osaka U (Addgene #51970)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

eNpHR3.0-EYFP was first inserted using BamHI/XhoI sites and then Nano-lantern was inserted using NotI/XhoI sites by replacing the Kir1.8 trafficking signal and the EYFP tag. The ER export motif (FCYENEV) was added at the C-terminal.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-iLMO2 was a gift from Robert Gross (Addgene plasmid # 129474 ; http://n2t.net/addgene:129474 ; RRID:Addgene_129474)

  • For your References section:

    Inhibitory luminopsins: genetically-encoded bioluminescent opsins for versatile, scalable, and hardware-independent optogenetic inhibition. Tung JK, Gutekunst CA, Gross RE. Sci Rep. 2015 Sep 24;5:14366. doi: 10.1038/srep14366. 10.1038/srep14366 PubMed 26399324
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