FUGAW
(Plasmid
#129473)
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Purpose3rd-generation lentiviral vector for hUbC-driven expression of GFP-aequorin
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 129473 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneFUGW
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Backbone manufacturerDavid Baltimore (Addgene plasmid # 14883)
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBleomycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGFP-aequorin
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SpeciesAequoria victoria
- Promoter human ubiquitin C
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FUGAW was a gift from Robert Gross (Addgene plasmid # 129473 ; http://n2t.net/addgene:129473 ; RRID:Addgene_129473) -
For your References section:
Bioluminescence imaging in live cells and animals. Tung JK, Berglund K, Gutekunst CA, Hochgeschwender U, Gross RE. Neurophotonics. 2016 Apr;3(2):025001. doi: 10.1117/1.NPh.3.2.025001. Epub 2016 Apr 5. 10.1117/1.NPh.3.2.025001 PubMed 27226972