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AAVS1-TRE3G-NFIA+SOX9
(Plasmid #129455)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129455 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAVS1-TRE3G
  • Backbone size w/o insert (bp) 11159
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sox 9
  • Alt name
    CMD1; SRA1; CMPD1; SRXX2; SRXY10
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1530
  • GenBank ID
    6662
  • Entrez Gene
    SOX9 (a.k.a. CMD1, CMPD1, SRA1, SRXX2, SRXY10)
  • Promoter TRE3G

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (unknown if destroyed)
  • 3′ cloning site MluI (unknown if destroyed)
  • 5′ sequencing primer ACCCGCACTTGCACAACG
  • 3′ sequencing primer GGTGGTCCTTCTTGTGCTG
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAVS1-TRE3G-NFIA+SOX9 was a gift from Su-Chun Zhang (Addgene plasmid # 129455 ; http://n2t.net/addgene:129455 ; RRID:Addgene_129455)

  • For your References section:

    Fast Generation of Functional Subtype Astrocytes from Human Pluripotent Stem Cells. Li X, Tao Y, Bradley R, Du Z, Tao Y, Kong L, Dong Y, Jones J, Yan Y, Harder CRK, Friedman LM, Bilal M, Hoffmann B, Zhang SC. Stem Cell Reports. 2018 Oct 9;11(4):998-1008. doi: 10.1016/j.stemcr.2018.08.019. Epub 2018 Sep 27. 10.1016/j.stemcr.2018.08.019 PubMed 30269954
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