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px335 Slc1a3 nickase 1
(Plasmid #129410)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129410 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pX335
  • Total vector size (bp) 8437
  • Vector type
    Mammalian Expression, Mouse Targeting, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgRNA directing Cas9n to the proximity of Slc1a3 STOP codon
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Slc1a3 (a.k.a. B430115D02Rik, Eaat1, GLAST, GLAST-1, GLU-T, GluT-1, Gmt1, MGluT1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (destroyed during cloning)
  • 3′ cloning site BbsI (destroyed during cloning)
  • 5′ sequencing primer U6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    px335 Slc1a3 nickase 1 was a gift from Walker Jackson (Addgene plasmid # 129410 ; http://n2t.net/addgene:129410 ; RRID:Addgene_129410)

  • For your References section:

    Slc1a3-2A-CreERT2 mice reveal unique features of Bergmann glia and augment a growing collection of Cre drivers and effectors in the 129S4 genetic background. Kaczmarczyk L, Reichenbach N, Blank N, Jonson M, Dittrich L, Petzold GC, Jackson WS. Sci Rep. 2021 Mar 8;11(1):5412. doi: 10.1038/s41598-021-84887-2. 10.1038/s41598-021-84887-2 PubMed 33686166
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