Slc1a3-CreERT2 Targeting Vector
(Plasmid
#129409)
-
PurposeGene targeting vector to knock-in 2A-CreERT2 into the mouse Slc1a3 locus in place of a STOP codon
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 129409 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepBlueScript
- Total vector size (bp) 11426
-
Vector typeMammalian Expression, Mouse Targeting, Cre/Lox
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameSlc1a3-2A-CreERT2 Targeting Vector
-
SpeciesM. musculus (mouse)
-
Entrez GeneSlc1a3 (a.k.a. B430115D02Rik, Eaat1, GLAST, GLAST-1, GLU-T, GluT-1, Gmt1, MGluT1)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
Slc1a3-CreERT2 Targeting Vector was a gift from Walker Jackson (Addgene plasmid # 129409 ; http://n2t.net/addgene:129409 ; RRID:Addgene_129409) -
For your References section:
Slc1a3-2A-CreERT2 mice reveal unique features of Bergmann glia and augment a growing collection of Cre drivers and effectors in the 129S4 genetic background. Kaczmarczyk L, Reichenbach N, Blank N, Jonson M, Dittrich L, Petzold GC, Jackson WS. Sci Rep. 2021 Mar 8;11(1):5412. doi: 10.1038/s41598-021-84887-2. 10.1038/s41598-021-84887-2 PubMed 33686166