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pLL1275
(Plasmid #129386)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129386 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLL1270
  • Backbone size w/o insert (bp) 7210
  • Total vector size (bp) 15573
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    alpha-Xylp_1211 (Ga0256695_ 1211)
  • Species
    LL1355
  • Insert Size (bp)
    1980
  • GenBank ID
    NZ_RSDX01000001
  • Promoter Clostridium thermocellum DSM1313 Cellobiose phosphorylase (CBP) Clo1313_1954

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TTATAGAGCTTAGGGAGAGG
  • 3′ sequencing primer CTGTCAAATATCGCCTTC
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    beta-Xylp_1710
  • Species
    LL1355
  • Insert Size (bp)
    2037
  • GenBank ID
    NZ_RSDX01000001
  • Promoter Clostridium thermocellum DSM1313 Cellobiose phosphorylase (CBP) Clo1313_1954

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TTAGGGAAAACAGACAGG
  • 3′ sequencing primer GGTGTGCTTATTTGGTGT
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    alpha-L-AraF_1120
  • Species
    LL1355
  • Insert Size (bp)
    1803
  • GenBank ID
    NZ_RSDX01000001
  • Promoter Clostridium thermocellum DSM1313 Cellobiose phosphorylase (CBP) Clo1313_1954

Cloning Information for Gene/Insert 3

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ACCTTTTGCCCTGATAAC
  • 3′ sequencing primer GTTAGCCTCCGTCCTTATT
    • (Common Sequencing Primers)

Gene/Insert 4

  • Gene/Insert name
    alpha-L-Galp_697
  • Species
    LL1355
  • Insert Size (bp)
    2475
  • GenBank ID
    NZ_RSDX01000001
  • Promoter Clostridium thermocellum DSM1313 Cellobiose phosphorylase (CBP) Clo1313_1954

Cloning Information for Gene/Insert 4

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GGATTTGTGGAGTTCTTG
  • 3′ sequencing primer TGTCCTTCTCTACTCCTCC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There exist mismatches compared to the expected reference that are not of functional concern to the. plasmid. These include T346A in alpha-Xylp_1211, D547G in beta-Xylp_1710, E565K, N566K, an indel that alters the end of the frame in alpha-L-Galp_697, and mismatches in AmpR.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLL1275 was a gift from Lee Lynd (Addgene plasmid # 129386 ; http://n2t.net/addgene:129386 ; RRID:Addgene_129386)
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