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Addgene

pLL1274
(Plasmid #129385)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129385 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLL1270
  • Backbone size w/o insert (bp) 7210
  • Total vector size (bp) 10631
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    alpha-Xylp_1211 (Ga0256695_ 1211)
  • Species
    LL1355
  • Insert Size (bp)
    1980
  • GenBank ID
    NZ_RSDX01000001
  • Promoter Clostridium thermocellum DSM1313 Cellobiose phosphorylase (CBP) Clo1313_1954

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TTATAGAGCTTAGGGAGAGG
  • 3′ sequencing primer CTGTCAAATATCGCCTTC
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    alpha-L-AraF_996 (Ga0256695_ 0996)
  • Species
    LL1355
  • Insert Size (bp)
    1418
  • GenBank ID
    NZ_RSDX01000001
  • Promoter Clostridium thermocellum DSM1313 Cellobiose phosphorylase (CBP) Clo1313_1954

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer AGGGTAAAGGCGAATATG
  • 3′ sequencing primer TACTATTGAACGCCTGCT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLL1274 was a gift from Lee Lynd (Addgene plasmid # 129385 ; http://n2t.net/addgene:129385 ; RRID:Addgene_129385)
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