pT2MP.EF-Q2m
(Plasmid
#129310)
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PurposeQUEEN ATP sensor for 25degC, Tol2 transposon
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 129310 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepT2+MCS
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameQUEEN-2m
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Depositor Adm #1027
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pT2MP.EF-Q2m was a gift from Yasushi Okada (Addgene plasmid # 129310 ; http://n2t.net/addgene:129310 ; RRID:Addgene_129310) -
For your References section:
Diversity in ATP concentrations in a single bacterial cell population revealed by quantitative single-cell imaging. Yaginuma H, Kawai S, Tabata KV, Tomiyama K, Kakizuka A, Komatsuzaki T, Noji H, Imamura H. Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038/srep06522. 10.1038/srep06522 PubMed 25283467