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Addgene

pDONR223 ATG3 allKR
(Plasmid #129295)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129295 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR223
  • Backbone size w/o insert (bp) 2793
  • Vector type
    Gateway Entry Vector / Entry clone

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ubiquitin-like-conjugating enzyme ATG3
  • Species
    H. sapiens (human)
  • Mutation
    changed all 22 Lysine residues to Arginine
  • Entrez Gene
    ATG3 (a.k.a. APG3, APG3-LIKE, APG3L, PC3-96, hApg3)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR223 ATG3 allKR was a gift from Robin Ketteler (Addgene plasmid # 129295 ; http://n2t.net/addgene:129295 ; RRID:Addgene_129295)

  • For your References section:

    Human ATG4 autophagy proteases counteract attachment of ubiquitin-like LC3/GABARAP proteins to other cellular proteins. Agrotis A, von Chamier L, Oliver H, Kiso K, Singh T, Ketteler R. J Biol Chem. 2019 Jul 17. pii: AC119.009977. doi: 10.1074/jbc.AC119.009977. 10.1074/jbc.AC119.009977 PubMed 31315929
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