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pDEST-CMV GFP-LC3B Q116P G120
(Plasmid #129290)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129290 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST-CMV-N-EGFP
  • Backbone size w/o insert (bp) 6052
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Microtubule-associated proteins 1A/1B light chain 3B
  • Species
    H. sapiens (human)
  • Mutation
    Changed Glutamine 116 to Proline. Deleted amino acids 121-125 (stop codon after G120).
  • GenBank ID
    NM_022818.4
  • Entrez Gene
    MAP1LC3B (a.k.a. ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST-CMV GFP-LC3B Q116P G120 was a gift from Robin Ketteler (Addgene plasmid # 129290 ; http://n2t.net/addgene:129290 ; RRID:Addgene_129290)

  • For your References section:

    Human ATG4 autophagy proteases counteract attachment of ubiquitin-like LC3/GABARAP proteins to other cellular proteins. Agrotis A, von Chamier L, Oliver H, Kiso K, Singh T, Ketteler R. J Biol Chem. 2019 Jul 17. pii: AC119.009977. doi: 10.1074/jbc.AC119.009977. 10.1074/jbc.AC119.009977 PubMed 31315929
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