pLL1270
(Plasmid
#129281)
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Purpose(Empty Backbone) The vector used for enzyme expression in Thermoanaerobacterium. thermosaccharolyticum throughout this study. Contains the CBP promoter from Clostridium thermocellum
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 129281 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLL1270
- Backbone size (bp) 7210
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Vector typeBacterial Expression
- Promoter Clostridium thermocellum DSM1313 Cellobiose Phosphorylase promoter Clo1313_1954
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLL1270 was a gift from Lee Lynd (Addgene plasmid # 129281 ; http://n2t.net/addgene:129281 ; RRID:Addgene_129281)
Map uploaded by the depositor.
