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PurposeExpression of APEX2 at nuclear pore
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 129276 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLX304
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameV5-APEX2-SENP2
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SpeciesH. sapiens (human)
- Promoter CMV promoter
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV forward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
V5-APEX2-SENP2 was a gift from Alice Ting (Addgene plasmid # 129276 ; http://n2t.net/addgene:129276 ; RRID:Addgene_129276) -
For your References section:
Atlas of Subcellular RNA Localization Revealed by APEX-Seq. Fazal FM, Han S, Parker KR, Kaewsapsak P, Xu J, Boettiger AN, Chang HY, Ting AY. Cell. 2019 Jun 14. pii: S0092-8674(19)30555-0. doi: 10.1016/j.cell.2019.05.027. 10.1016/j.cell.2019.05.027 PubMed 31230715
