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PurposeExpression of APEX2 in the nucleolus
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 129274 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLX304
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGFP-APEX2-NIK3x
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SpeciesH. sapiens (human)
- Promoter CMV promoter
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV forward (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GFP-APEX2-NIK3x was a gift from Alice Ting (Addgene plasmid # 129274 ; http://n2t.net/addgene:129274 ; RRID:Addgene_129274) -
For your References section:
Atlas of Subcellular RNA Localization Revealed by APEX-Seq. Fazal FM, Han S, Parker KR, Kaewsapsak P, Xu J, Boettiger AN, Chang HY, Ting AY. Cell. 2019 Jun 14. pii: S0092-8674(19)30555-0. doi: 10.1016/j.cell.2019.05.027. 10.1016/j.cell.2019.05.027 PubMed 31230715