pBBR5pemIKpKan
(Plasmid
#129271)
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Purpose(Empty Backbone) Broad host-range plasmid with toxin-antitoxin system for maintenance in the absence of antibiotic selection, and constitutive promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 129271 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBBR1-MCS5
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Modifications to backbonepemI/pemK plasmid stability factor Gateway cloning cassette constitutive promoter
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberLow Copy
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer see supplemental info for primer sequence
- 3′ sequencing primer M13 rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBBR5pemIKpKan was a gift from Lindsey Burbank (Addgene plasmid # 129271 ; http://n2t.net/addgene:129271 ; RRID:Addgene_129271) -
For your References section:
Broad-Host-Range Plasmids for Constitutive and Inducible Gene Expression in the Absence of Antibiotic Selection. Burbank L, Wei W. Microbiol Resour Announc. 2019 Sep 5;8(36). pii: 8/36/e00769-19. doi: 10.1128/MRA.00769-19. 10.1128/MRA.00769-19 PubMed 31488530
Map uploaded by the depositor.
