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pGAN213
(Plasmid #129270)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129270 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGAN147
  • Backbone manufacturer
    Gregory Newby
  • Total vector size (bp) 7233
  • Modifications to backbone
    Removed synTA expression cassette
  • Vector type
    Yeast Expression
  • Selectable markers
    Nourseothricin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mNeonGreen
  • Species
    Synthetic
  • Insert Size (bp)
    702
  • Promoter min pCYC1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site DraIII (destroyed during cloning)
  • 3′ cloning site PacI (destroyed during cloning)
  • 5′ sequencing primer cagcactaaagttgcctggc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

reporter only (no yTRAP fusion). 8xop-crCYC1p-NeonGreen-ADH1term.
for measuring background levels of reporter protein

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGAN213 was a gift from Ahmad Khalil (Addgene plasmid # 129270 ; http://n2t.net/addgene:129270 ; RRID:Addgene_129270)

  • For your References section:

    A Genetic Tool to Track Protein Aggregates and Control Prion Inheritance. Newby GA, Kiriakov S, Hallacli E, Kayatekin C, Tsvetkov P, Mancuso CP, Bonner JM, Hesse WR, Chakrabortee S, Manogaran AL, Liebman SW, Lindquist S, Khalil AS. Cell. 2017 Nov 2;171(4):966-979.e18. doi: 10.1016/j.cell.2017.09.041. Epub 2017 Oct 19. 10.1016/j.cell.2017.09.041 PubMed 29056345
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