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pCAG-Mac_Q139D_D150N_E258V-HaloTag
(Plasmid #129259)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129259 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mac_Q139D_D150N_E258V-HaloTag
  • Species
    Synthetic
  • Mutation
    Q139D,D150N,E258V
  • Promoter CAG

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

5' cloning site: NheI, 3' cloning site: HindIII.
Please visit https://www.biorxiv.org/content/10.1101/690925v1.full to read the preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-Mac_Q139D_D150N_E258V-HaloTag was a gift from Eric Schreiter (Addgene plasmid # 129259 ; http://n2t.net/addgene:129259 ; RRID:Addgene_129259)

  • For your References section:

    A general approach to engineer positive-going eFRET voltage indicators. Abdelfattah AS, Valenti R, Zheng J, Wong A, Podgorski K, Koyama M, Kim DS, Schreiter ER. Nat Commun. 2020 Jul 10;11(1):3444. doi: 10.1038/s41467-020-17322-1. 10.1038/s41467-020-17322-1 PubMed 32651384
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