pGEX-4T-1-3xMyc-ERK2(K52R)
(Plasmid #129228)

• Purpose: Encodes Myc tagged human ERK2(K52R) for purification by GST affinity
• Depositing Lab: Kevin Janes
• Publication: Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10.1038/s41598-019-55998-8.

Backbone

• Vector backbone: pGEX-4T1
• Backbone manufacturer: Amersham
• Backbone size w/o insert (bp): 4969
• Total vector size (bp): 6135
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ERK2(K52R)
• Species: R. norvegicus (rat)
• Insert Size (bp): 1179
• Mutation: Changed Lysine 52 to Arginine
• GenBank ID: NM_053842
• Entrez Gene: Mapk1 (a.k.a. ERK-2, ERT1, Erk2, p42-MAPK)
• Tags / Fusion Proteins:
  • GST (N terminal on backbone)
  • Myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: 5GEX
• 3′ sequencing primer: 3GEX

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Depositor uses BL21 for protein production.