pGEX-4T-1-3xHA-IκBα(1-62)
(Plasmid
#129226)
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PurposeEncodes HA tagged human IκBα(1-62) for purification by GST affinity
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 129226 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX-4T1
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Backbone manufacturerAmersham
- Backbone size w/o insert (bp) 4969
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameIkBa(1-62)
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Alt nameNFKBIA
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SpeciesH. sapiens (human)
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Insert Size (bp)201
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GenBank IDNM_020529
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Entrez GeneNFKBIA (a.k.a. EDAID2, IKBA, MAD-3, NFKBI)
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Tags
/ Fusion Proteins
- GST (N terminal on backbone)
- HA (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer 5GEX
- 3′ sequencing primer 3GEX (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Depositor uses BL21 for protein production.
There is a single mutation in LacI- L189S, but this does not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-4T-1-3xHA-IκBα(1-62) was a gift from Kevin Janes (Addgene plasmid # 129226 ; http://n2t.net/addgene:129226 ; RRID:Addgene_129226) -
For your References section:
An ultrasensitive fiveplex activity assay for cellular kinases. Smolko CM, Janes KA. Sci Rep. 2019 Dec 19;9(1):19409. doi: 10.1038/s41598-019-55998-8. 10.1038/s41598-019-55998-8 PubMed 31857650