pGAN201
(Plasmid #129204)

• Purpose: yTRAP sensor of [RNQ+], detects aggregation of Rnq1
• Depositing Lab: Ahmad Khalil
• Publication: Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi: 10.1016/j.cell.2017.09.041. Epub 2017 Oct 19.

Backbone

• Vector backbone: pGAN147
• Backbone manufacturer: Gregory Newby
• Backbone size w/o insert (bp): 8600
• Total vector size (bp): 10259
• Modifications to backbone: LR reaction with pENTR-Rnq1
• Vector type: Yeast Expression, Synthetic Biology
• Selectable markers: Nourseothricin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RNQ1
• Alt name: YCL028W
• Species: S. cerevisiae (budding yeast), Synthetic
• Insert Size (bp): 4000
• GenBank ID: NM_001178673.1
• Entrez Gene: RNQ1 (a.k.a. YCL028W)
• Promoter: SUP35

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: Unknown
• 3′ sequencing primer: Unknown

Resource Information

• Supplemental Documents:
  • pGAN201_RNQ1 yTRAP.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is the basic yTRAP sensor to sense aggregation of Rnq1 or [RNQ+]. It should be linearized with NotI-HF enzyme prior to integration into the yeast genome. The sensor integrates into the HO locus, and can be selected for using nourseothricin.