pGAN147
(Plasmid #129202)

• Purpose: (Empty Backbone) yeast transcriptional reporter of aggregating proteins (yTRAP) to detect protein aggregation, Gateway backbone
• Depositing Lab: Ahmad Khalil
• Publication: Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi: 10.1016/j.cell.2017.09.041. Epub 2017 Oct 19.

Backbone

• Vector backbone: pDML112
• Backbone manufacturer: Dirk Landgraf
• Backbone size (bp): 5056
• Vector type: Yeast Expression, Synthetic Biology
• Promoter: SUP35
• Selectable markers: Nourseothricin

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Growth instructions: DB3.1
• Copy number: High Copy

Resource Information

• Supplemental Documents:
  • GAN147-NAT-Sup-Neon-43-8.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is the empty basic yTRAP sensor plasmid. To sense aggregation of your favorite protein, clone the coding sequence into a pENTR clone, then perform LR reaction with pGAN147. The resulting expression clone should be linearized with NotI-HF enzyme prior to integration into the yeast genome. The sensor integrates into the HO locus, and can be selected for using nourseothricin.