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Addgene

pcDNA-GATA2-DCzf
(Plasmid #1292)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1292 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GATA2
  • Alt name
    GATA-2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1400
  • GenBank ID
    NM_002050
  • Entrez Gene
    GATA2 (a.k.a. DCML, IMD21, MONOMAC, NFE1B)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (destroyed during cloning)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Amino acids 342-367 in GATA-2 were deleted in the DCzf mutant to remove the carboxyl zinc finger.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-GATA2-DCzf was a gift from Gokhan Hotamisligil (Addgene plasmid # 1292 ; http://n2t.net/addgene:1292 ; RRID:Addgene_1292)

  • For your References section:

    Function of GATA transcription factors in preadipocyte-adipocyte transition. Tong Q, Dalgin G, Xu H, Ting CN, Leiden JM, Hotamisligil GS. Science 2000 Oct 6;290(5489):134-8. 10.1126/science.290.5489.134 PubMed 11021798
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