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pBADHISTEV_PfeEH258A
(Plasmid #129122)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129122 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBADHISTEV
  • Backbone size w/o insert (bp) 4627
  • Total vector size (bp) 5443
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Periplasmic Esterase PfeE
  • Alt name
    PA2689
  • Alt name
    PfeE
  • Species
    Pseudomonas aeruginosa PAO1
  • Mutation
    Signal peptide sequence has been removed, mutation histidine 258 to alanine
  • Entrez Gene
    PA2689 (a.k.a. PA2689)
  • Promoter PBAD
  • Tag / Fusion Protein
    • TEV protease cleavable 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI/BspHI (destroyed during cloning)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CTTTGCTATGCCATAGC
  • 3′ sequencing primer TTTCACTTCTGAGTTCG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pBADHISTEV has been provided by Huanting Liu

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBADHISTEV_PfeEH258A was a gift from James Naismith (Addgene plasmid # 129122 ; http://n2t.net/addgene:129122 ; RRID:Addgene_129122)

  • For your References section:

    A Key Role for the Periplasmic PfeE Esterase in Iron Acquisition via the Siderophore Enterobactin in Pseudomonas aeruginosa. Perraud Q, Moynie L, Gasser V, Munier M, Godet J, Hoegy F, Mely Y, Mislin GLA, Naismith JH, Schalk IJ. ACS Chem Biol. 2018 Sep 21;13(9):2603-2614. doi: 10.1021/acschembio.8b00543. Epub 2018 Aug 22. 10.1021/acschembio.8b00543 PubMed 30086222
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