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Addgene

pGRCRE
(Plasmid #129116)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129116 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCg2XpH-N
  • Modifications to backbone
    Two pHluorins are replaced with cre recombinase encoding gene under S. cerevisiae GAL1 promoter
  • Vector type
    Yeast Expression, Cre/Lox
  • Selectable markers
    nourseothricin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    cre
  • Alt name
    cre recombinase
  • Promoter S. cerevisiae GAL1 promoter

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: Plasmid contains 62 additional basepairs in the backbone between the f1 ori and C. glabrata ACT1 feature compared to the depositor's sequence. This difference is not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGRCRE was a gift from Paolo Giudici (Addgene plasmid # 129116 ; http://n2t.net/addgene:129116 ; RRID:Addgene_129116)

  • For your References section:

    A set of plasmids carrying antibiotic resistance markers and Cre recombinase for genetic engineering of nonconventional yeast Zygosaccharomyces rouxii. Bizzarri M, Cassanelli S, Duskova M, Sychrova H, Solieri L. Yeast. 2019 Dec;36(12):711-722. doi: 10.1002/yea.3438. Epub 2019 Sep 11. 10.1002/yea.3438 PubMed 31414502
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