pZEG
(Plasmid
#129112)
-
Purpose(Empty Backbone) Z. rouxii episomal vector containing G418 resistance
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 129112 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepZEU
-
Modifications to backboneloxP-KanMX-loxP cassette conferring G418 resistance instead of URA3 marker
-
Vector typeYeast Expression, Cre/Lox
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Cloning Information
- Cloning method Unknown
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pZEG was a gift from Paolo Giudici (Addgene plasmid # 129112 ; http://n2t.net/addgene:129112 ; RRID:Addgene_129112) -
For your References section:
A set of plasmids carrying antibiotic resistance markers and Cre recombinase for genetic engineering of nonconventional yeast Zygosaccharomyces rouxii. Bizzarri M, Cassanelli S, Duskova M, Sychrova H, Solieri L. Yeast. 2019 Dec;36(12):711-722. doi: 10.1002/yea.3438. Epub 2019 Sep 11. 10.1002/yea.3438 PubMed 31414502