pETM6_Ptrc_BsMdh_BmHps_BmPhi_Pthl_CaThl_CaCtfAB_CaAdc
(Plasmid
#129105)
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PurposeExpresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus as well as Clostridium acetobutylicum genes required for Acetate-dependent Acetone production
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 129105 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepETM6
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBsMDH BmHPS BmPHI CaTHL CaCtfAB CaADC
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GenBank IDBSU_29120, PB1_00480, PB1_00485 CA_P0078, CA_P0163, CA_P0164, CA_P0165
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pETM6_Ptrc_BsMdh_BmHps_BmPhi_Pthl_CaThl_CaCtfAB_CaAdc was a gift from Eleftherios Terry Papoutsakis (Addgene plasmid # 129105 ; http://n2t.net/addgene:129105 ; RRID:Addgene_129105) -
For your References section:
Expression of heterologous non-oxidative pentose phosphate pathway from Bacillus methanolicus and phosphoglucose isomerase deletion improves methanol assimilation and metabolite production by a synthetic Escherichia coli methylotroph. Bennett RK, Gonzalez JE, Whitaker WB, Antoniewicz MR, Papoutsakis ET. Metab Eng. 2018 Jan;45:75-85. doi: 10.1016/j.ymben.2017.11.016. Epub 2017 Dec 5. 10.1016/j.ymben.2017.11.016 PubMed 29203223