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pETM6_Ptrc_BsMdh_BmHps_BmPhi
(Plasmid #129104)

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Item Catalog # Description Quantity Price (USD)
Plasmid 129104 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pETM6
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BsMDH BmHPS BmPHI
  • Species
    Bacillus subtilis and Bacillus methanolicus
  • GenBank ID
    BSU_29120, PB1_00480, PB1_00485

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETM6_Ptrc_BsMdh_BmHps_BmPhi was a gift from Eleftherios Terry Papoutsakis (Addgene plasmid # 129104 ; http://n2t.net/addgene:129104 ; RRID:Addgene_129104)

  • For your References section:

    Expression of heterologous non-oxidative pentose phosphate pathway from Bacillus methanolicus and phosphoglucose isomerase deletion improves methanol assimilation and metabolite production by a synthetic Escherichia coli methylotroph. Bennett RK, Gonzalez JE, Whitaker WB, Antoniewicz MR, Papoutsakis ET. Metab Eng. 2018 Jan;45:75-85. doi: 10.1016/j.ymben.2017.11.016. Epub 2017 Dec 5. 10.1016/j.ymben.2017.11.016 PubMed 29203223
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