pETM6_Ptac_BmHps_BmPhi_BsMdh
(Plasmid
#129103)
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PurposeExpresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 129103 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepETM6
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBsMDH BmHPS BmPHI
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GenBank IDBSU_29120, PB1_00480, PB1_00485
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pETM6_Ptac_BmHps_BmPhi_BsMdh was a gift from Eleftherios Terry Papoutsakis (Addgene plasmid # 129103 ; http://n2t.net/addgene:129103 ; RRID:Addgene_129103) -
For your References section:
Engineering the biological conversion of methanol to specialty chemicals in Escherichia coli. Whitaker WB, Jones JA, Bennett RK, Gonzalez JE, Vernacchio VR, Collins SM, Palmer MA, Schmidt S, Antoniewicz MR, Koffas MA, Papoutsakis ET. Metab Eng. 2017 Jan;39:49-59. doi: 10.1016/j.ymben.2016.10.015. Epub 2016 Nov 1. 10.1016/j.ymben.2016.10.015 PubMed 27815193