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Addgene

pQC-CherryOFF-GFP
(Plasmid #129101)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129101 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQC-XIG
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 7116
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CherryOFF
  • Alt name
    GFP
  • Species
    Synthetic
  • Mutation
    G-> A
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene plasmid # 26286 is the empty vector negative control.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQC-CherryOFF-GFP was a gift from Fangliang Zhang (Addgene plasmid # 129101 ; http://n2t.net/addgene:129101 ; RRID:Addgene_129101)

  • For your References section:

    A Rapid and Precise Mutation-Activated Fluorescence Reporter for Analyzing Acute Mutagenesis Frequency. Birnbaum MD, Nemzow L, Kumar A, Gong F, Zhang F. Cell Chem Biol. 2018 Aug 16;25(8):1038-1049.e5. doi: 10.1016/j.chembiol.2018.05.010. Epub 2018 Jun 14. 10.1016/j.chembiol.2018.05.010 PubMed 29909992
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