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PurposeFor tagging genes at 3'end (C-terminal end of protein) in S. cerevisiae with mNeonGreen
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 129099 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFA6a
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Vector typeYeast Expression
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Selectable markersKanamycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemNeonGreen
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SpeciesBranchiostoma lanceolatum
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Insert Size (bp)708
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site PacI (not destroyed)
- 3′ cloning site AscI (not destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
To design forward PCR primer for C-terminal tagging, use: 5'-(gene-specific sequence) ATGGTGAGTAAAGGCGAGGA-3'. To design reverse PCR primer for C-terminal tagging, use: 5'-(gene-specific sequence) GAATTCGAGCTCGTTTAAAC-3'.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFA6a-mNeonGreen-KanMX6 was a gift from Wei-Lih Lee (Addgene plasmid # 129099 ; http://n2t.net/addgene:129099 ; RRID:Addgene_129099)
