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Addgene

GFP-HDEL
(Plasmid #129098)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129098 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS306
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TEF1p-SS-3xGlyAla-GFP-HDEL
  • Species
    Synthetic
  • Insert Size (bp)
    1304

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Linearize the plasmid with StuI for integration into URA3 locus.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-HDEL was a gift from Wei-Lih Lee (Addgene plasmid # 129098 ; http://n2t.net/addgene:129098 ; RRID:Addgene_129098)

  • For your References section:

    Cortical dynein pulling mechanism is regulated by differentially targeted attachment molecule Num1. Omer S, Greenberg SR, Lee WL. Elife. 2018 Aug 7;7. pii: 36745. doi: 10.7554/eLife.36745. 10.7554/eLife.36745 PubMed 30084355
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