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pCR2.1-KRAS-G12V
(Plasmid #129091)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129091 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR2.1-TOPO cloning vector
  • Vector type
    homologous recombination donor plasmid
  • Selectable markers
    KRAS(G12V)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KRAS(G12V)
  • Species
    H. sapiens (human)
  • Mutation
    G12V
  • Entrez Gene
    KRAS (a.k.a. 'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCR2.1-KRAS-G12V was a gift from Toshiro Sato (Addgene plasmid # 129091 ; http://n2t.net/addgene:129091 ; RRID:Addgene_129091)

  • For your References section:

    Modeling colorectal cancer using CRISPR-Cas9-mediated engineering of human intestinal organoids. Matano M, Date S, Shimokawa M, Takano A, Fujii M, Ohta Y, Watanabe T, Kanai T, Sato T. Nat Med. 2015 Mar;21(3):256-62. doi: 10.1038/nm.3802. Epub 2015 Feb 23. 10.1038/nm.3802 PubMed 25706875
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