pGEX-5X-1 SmB-N
(Plasmid
#129075)
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Purposegeneration of GST-tagged N-terminal part of SmB protein using bacteria
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 129075 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX-5X-1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSmB
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SpeciesH. sapiens (human)
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Mutationcontains amino acids 1-492
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Entrez GeneSNRPB (a.k.a. CCMS, COD, SNRPB1, Sm-B/B', SmB/B', SmB/SmB', snRNP-B)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-5X-1 SmB-N was a gift from Takeshi Tsubata (Addgene plasmid # 129075 ; http://n2t.net/addgene:129075 ; RRID:Addgene_129075)