pET28aAviHisCD72aCTLD
(Plasmid
#129065)
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Purposegeneration of biotinylated mouse CD72a CTLD protein using bacteria
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 129065 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28a
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCD72a
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SpeciesM. musculus (mouse)
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Entrez GeneCd72 (a.k.a. CD72c, Ly-19, Ly-32, Ly-m19, Lyb-2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28aAviHisCD72aCTLD was a gift from Takeshi Tsubata (Addgene plasmid # 129065 ; http://n2t.net/addgene:129065 ; RRID:Addgene_129065) -
For your References section:
CD72 negatively regulates B lymphocyte responses to the lupus-related endogenous toll-like receptor 7 ligand Sm/RNP. Akatsu C, Shinagawa K, Numoto N, Liu Z, Ucar AK, Aslam M, Phoon S, Adachi T, Furukawa K, Ito N, Tsubata T. J Exp Med. 2016 Nov 14;213(12):2691-2706. doi: 10.1084/jem.20160560. Epub 2016 Oct 24. 10.1084/jem.20160560 PubMed 27810925