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Addgene

pMM0657
(Plasmid #129018)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129018 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    NA
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ConL1-pRPL18B-SV40NLS-ZiF268DBD-CRY2-tSSA1-ConR2-ColE1-AmpR
  • Species
    S. cerevisiae (budding yeast)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMM0657 was a gift from Megan McClean (Addgene plasmid # 129018 ; http://n2t.net/addgene:129018 ; RRID:Addgene_129018)

  • For your References section:

    A yeast optogenetic toolkit (yOTK) for gene expression control in Saccharomyces cerevisiae. An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes BL, Noyes MB, McClean MN. Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234. 10.1002/bit.27234 PubMed 31788779
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