pMM0554
(Plasmid
#128990)
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PurposeLEU2 integrtion vector for VP16-CIB1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 128990 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMM494
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLeu2 5' homology-pTDH3-SV40NLS-VP16-CIB1-tADH1-Con1-LEU2-Leu2 3' homology
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SpeciesS. cerevisiae (budding yeast)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMM0554 was a gift from Megan McClean (Addgene plasmid # 128990 ; http://n2t.net/addgene:128990 ; RRID:Addgene_128990) -
For your References section:
A yeast optogenetic toolkit (yOTK) for gene expression control in Saccharomyces cerevisiae. An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes BL, Noyes MB, McClean MN. Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234. 10.1002/bit.27234 PubMed 31788779