pTAMAHISTEV_Ab-PiuA
(Plasmid
#128957)
-
Purposeexpresses Acinetobacter baumannii PiuA in E. coli
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 128957 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepTAMAHISTEV
- Backbone size w/o insert (bp) 5566
- Total vector size (bp) 7704
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameTonB-dependent siderophore receptor
-
Alt nameA1S_0474
-
Alt nameAb-PiuA
-
SpeciesAcinetobacter baumannii ATCC 19606
-
Insert Size (bp)2359
-
MutationSignal peptide sequence has been removed
-
GenBank IDEEX03505.1
- Promoter T7
-
Tags
/ Fusion Proteins
- TEV protease cleavable 7xHis (N terminal on backbone)
- TamA (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pTAMAHISTEV vector has been obtained by replacing pelB signal peptide of pEPELBHISTEV (courtesy of Dr. Huanting Liu, University St Andrews) by tamA signal peptide
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pTAMAHISTEV_Ab-PiuA was a gift from James Naismith (Addgene plasmid # 128957 ; http://n2t.net/addgene:128957 ; RRID:Addgene_128957) -
For your References section:
Structure and Function of the PiuA and PirA Siderophore-Drug Receptors from Pseudomonas aeruginosa and Acinetobacter baumannii. Moynie L, Luscher A, Rolo D, Pletzer D, Tortajada A, Weingart H, Braun Y, Page MG, Naismith JH, Kohler T. Antimicrob Agents Chemother. 2017 Mar 24;61(4). pii: AAC.02531-16. doi: 10.1128/AAC.02531-16. Print 2017 Apr. 10.1128/AAC.02531-16 PubMed 28137795