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pET20b_Pa-PiuA
(Plasmid #128955)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 128955 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-20b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3716
  • Total vector size (bp) 5818
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    iron transport outer membrane receptor (PiuA)
  • Alt name
    PiuA
  • Alt name
    PA4514
  • Species
    Pseudomonas aeruginosa PAO1
  • Insert Size (bp)
    2160
  • Mutation
    Signal peptide sequence has been removed
  • Entrez Gene
    PA4514 (a.k.a. PA4514)
  • Promoter T7
  • Tags / Fusion Proteins
    • 6xHis (C terminal on backbone)
    • pelB leader (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BspHI (destroyed during cloning)
  • 3′ cloning site EagI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET20b_Pa-PiuA was a gift from James Naismith (Addgene plasmid # 128955 ; http://n2t.net/addgene:128955 ; RRID:Addgene_128955)

  • For your References section:

    Structure and Function of the PiuA and PirA Siderophore-Drug Receptors from Pseudomonas aeruginosa and Acinetobacter baumannii. Moynie L, Luscher A, Rolo D, Pletzer D, Tortajada A, Weingart H, Braun Y, Page MG, Naismith JH, Kohler T. Antimicrob Agents Chemother. 2017 Mar 24;61(4). pii: AAC.02531-16. doi: 10.1128/AAC.02531-16. Print 2017 Apr. 10.1128/AAC.02531-16 PubMed 28137795
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